Prevalence of High-Risk Antibiotic Resistant Acinetobacter baumannii in the Holy Cities of Makkah and Al-Madinah

Background: Acinetobacter baumannii strains resistant to carbapenems are a global public health problem. Objectives: The aim of the present study is evaluate prevalence genetic fingerprints associated with Metallo β-lactamases in A. addition clonal diversity Makkah and Al-Madinah regions Saudi Arabia, which receive high number international visitors. Methods: Multi-antibiotic isolates were investigated. Bacterial isolation was conducted employing basic bacteriological technique after confirming ID isolates. antimicrobial susceptibility test carried out using Vitek 2 compact system. molecular determined by Pulse Field Gel Electrophoresis (PFGE). Clusters analyzed BioNumerics software version 6.5. Dice coefficient used for calculating similarities. Results: results indicated resistance 82.5% against carbapenems. All found be sensitive colistin, while 5% tigecycline. screening carbapenem-resistant showed that dissemination imipenem meropenem 81 84%, respectively, majority susceptible tigecycline colistin. bla OXA VIM most encountered genes isolates, IS Aba1 prominent insertion sequence. fingerprinting (PFGE) revealed two types epidemic clones: monoclonal polyclonal models 17 clusters. Conclusion: current investigation indicates region clones present. It has also been demonstrated such create serious infection other parts world as heavy pilgrimage traffic received throughout year Al-Madinah, especially Haj season.